(Abstract); Kuci V, Nordström L, Jerkeman M, Ek S (2015) Emerging role of of 111In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.

av J Alfredsson Timmins · 2009 — forms of the HP1 proteins that perform slightly different functions (Lomberk et al., 2006 choring of the telomeres to the NM is facilitated by Ku70, Esc1, members of the Sir protein Hum Genet 80, 235-46. Cremer, T. and

The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. functions related to the stability of chromosomes in mammalian cells.7) Ku mutants have been phenotypically characterized in many organisms, including yeast, animal cells, plants, and fungi. In yeast Saccharomyces cerevisiae Ku Ku70/80 C-terminalarm; and positions 618 Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice AyakoNishizawa-Yokoi1,SatokoNonaka1,2,HiroakiSaika1,Yong-IkKwon1,3,KeishiOsakabe1,4 andSeiichiToki1,3 1Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan; 2Graduate … 2007-01-23 The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. Databases. EM maps have been deposited in EMDB (EMD‐11933). Coordinates have been deposited in Protein Data Bank Ku70 is known as the key component of classic NHEJ that plays a critical role in detecting DSBs (Puebla-Osorio et al., 2014).

Ku70 80 function

Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert. 2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig. 3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure. The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends. Work in our lab has shown that a D192A/D195R mutation in helix five of the Ku70 von Willebrand A (vWA) domain leads to an NHEJ defect. However, little is known about the function of this region in We propose a structural model of the functions of the subunit‐specific C‐terminal domains in the context of the full‐length Ku70–Ku80 protein .

With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research. 2013-02-06 · Ku70/80 has a toroid structure which fits over the DNA chain and is thought to provide a platform that enhances the binding of DNA-PKcs to the damaged DNA . It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model.

Regulation of telomere length by fatty acid elongase 3 in yeast: Involvement of inositol phosphate metabolism and Ku70/80 function Suriyan Ponnusamy, Nathan L. Alderson, Hiroko Hama, Jacek Bielawski, James C. Jiang, Rashna Bhandari, Solomon H. Snyder , S. Michal Jazwinski, Besim Ogretmen

The role of the ku80 gene in non-homologous end-joining (NHEJ) was investigated by calculating the frequency of homolo-gous recombination. The transformation test veriﬁed that Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Ku70 is known as the key component of classic NHEJ that plays a critical role in detecting DSBs (Puebla-Osorio et al., 2014). Thus, in the present study, we focused the specific role of Ku70 in DNA damage caused by F. nucleatum infection. However, another model is clearly needed to explore the role of Ku70/80 heterodimer under F. nucleatum

2012-02-10 2013-07-01 2004-07-09 KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor. Ku70, a known nonhomologous end-joining (NHEJ) factor, also functions in tumor suppression, although this molecular mechanism remains uncharacterized. It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994). Accordingly, these results may be attributed to a decrease in protein levels of both OsKu80 and OsKu70 caused by the down‐regulation of the expression of OsKu80 mRNA in KD‐Ku80 rice calli The Ku70/80 heterodimer binds to DNA ends with high a nity and speciﬁcity. The protein forms a ring that can thread onto a DNA end, which explains its inability to bind to circular DNA molecules or internal positions on the chromosome [5]. Once bound to DNA, Ku70/80 attracts the catalytic subunit of 2016-01-01 Several observations correlate Ku70/80 heterodimer function, DNA repair and tumor development (Difilippantonio et al., 2000), but no direct evidences of Ku70/80 activity in human tumor tissues The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis.

Ku70 and Ku80 (also known as XRCC6 and XRCC5, respectively) proteins, which can recruit DNA-PKcs and 25 May 2007 Summary: Subunit of the Ku70:Ku80 complex; binds RNA and damaged Composed in yeast of Yku70p and Yku80p, Ku functions in genome Ku70/Ku80 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA DNA-polymerase 3´ to 5´editing function removes incorrectly inserted nucleotides. Describe Proteinfaktorer som möjliggör detta är Ku70/80 och DNAks som av K Söderlund Leifler · 2009 — associated with cancer due to gain-of-function mutations are called proto- oncogenes binding to Ku70/80, DNA-PKcs is activated by autophosphorylation and. The corresponding antigen was identified as the Ku70/80 heterodimer, a nuclear been suggested to function in adhesion, migration and invasion processes. av J Fransson · 2006 — Functionally-associated target antigens in cancer.
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Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). 1999-01-01 Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818.

We have shown that Ku70/80 is recruited directly to all DSBs whereas DNA-PKcs is only recruited to longer-lived DSBs, which are suggested to be complex.
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(Abstract); Kuci V, Nordström L, Jerkeman M, Ek S (2015) Emerging role of of 111In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.

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